Soil Microbiotics is compatible with liquid fertilizers with a pH range of 5 to 9.

*Jar Test to assure compatibility with different mixtures of chemicals, liquid fertilizers and herbicides.  Do not mix with fungicides.

Mix in the following order: fertilizers, chemicals, water, and Soil MicroBiotics.

Clones

Pour a small portion of Green Microbiotics into a clean container.  Dip stems in undiluted Green Microbioitcs. Transplant into cubes or trays.

Soil and Soilless Mediums  

Premix 12ml-16ml of Green Microbiotics per gallon of water. Use you normal watering regiment. 

Flush Week

Discontinue Green Microbioitics

NOTE

Water usage may increase due to higher nutrient metabolism.  

Watch plants closely. Reduce nutrients if plants show signs of tip burning.

Hydroponic (Water Only)

Use 3 ml per gallon of water from clone through vegetative state

Use 5ml per gallon of water from flowering stage up to flush week

Flush week

Discontinue Green Microbioitics

NOTE

Watch plants closely. Reduce nutrients if plants show signs of tip burning

Add fungicides or pesticides separately.  Do not mix Green Microbioitics directly with fungicides or pesticides.

*Add fungicides or pesticides separately.  Do not mix Green Microbioitics directly with fungicides or pesticides.  

BIOREMEDIATION OF SALT CONTAMINATED SOIL

BIOREMEDIATION OF SALT CONTAMINATED SOIL

I. Preparation Phase

A. Soil Sample Collection

1. The samples will be a composite of 5 to 6 probes per site area.  Testing of soil and water by qualified and certified laboratory from sample taken according to the proper procedures to determine the extent and concentration of contamination.
2. The composite samples will include soil from the surface to the depth of contamination.
3. The composite sample should be submitted to an independent laboratory for analysis of the type of contaminants and extent of contamination.

B. Soil Preparation

1. The soil will be tilled to a depth of approximately 4-6 inches, and contaminations with depth greater than 12 inches should be bioremediated “in-situ” or excavated and contained and treated in lifts not to exceed 12 inches.
2. If the soil is dry, water will be added to bring moisture content to 50%-70% of field capacity.

II. Bioremediation Phase

Normally,  “produced water” spills will also have some hydrocarbon contamination.  Therefore, testing should assess both salt and hydrocarbon contamination.  The bioremediation phase will require 5 to 7 weeks with soil temperatures above 50 degrees Fahrenheit and below 87 degrees Fahrenheit.  Longer periods will be required if temperatures move outside of these parameters. 

Aeration is necessary due to the need for oxygen in the remediation process. When Dealing with a fresh spill, normal discing or other tillage methods are not possible, and Clean MicroBiotics and other products would be sprayed directly onto the contaminated area.  When possible, soil tillage would be beneficial to incorporate Clean MicroBiotics and other products into the soil.  Further aeration will be determined by the extents of the contamination is within acceptable levels.  Soil types may dictate more frequent tillage. Example: Clay soil will  need more tillage than sandy soil.

A. Application

Apply at a rate of:

1. Light contamination (Sodium: 100-200 ppm)

a.  .5 gallons Clean MicroBiotics / 1.85 cubic yards.

b.   5 gallons .5-0-0-3 Humic Acid/1.85 cubic yards.

2. Heavy contamination (Sodium: 3000 ppm or higher)

a.  .5 gallons Clean MicroBiotics / .50 cubic yards.

b.   10 gallons .5-0-0-3 Humic Acid/1.85 cubic yards.

3. Accelerator 92 – at an approximate rate of  2 gallons/ 100 gallons of Clean MicroBiotics.

4. .5-0-0-3 Humic Acid should be mixed with fresh water (preferably low iron content water) at an approximate rate of 4 gallons to 1 gallon of  .5-0-0-3 Humic Acid.

5. If high levels of hydrocarbon are present, a “combination treatment” is advised.  See BIOREMEDIATION OF HYDROCARBON CONTAMINATED SOIL

Soil moisture must be maintained at 50 – 70% of field capacity during the entire remediation period.

If necessary, a second application of Clean MicroBiotics with additional nutrients will be sprayed and incorporated into the soil surface.

III. Planting Phase

Soil will be prepared for planting five to seven weeks after the beginning of bioremediation phase.

A. Soil Sample Collection

Soil samples will be collected again as previously described.  They will be split and retested by both laboratories to confirm the bioremediation phase is complete.  Soil fertility application will be determined according to soil test results.

B. Seed Selection

A soil building perennial grass mixture will be selected that is adapted to the climate and soils of the area.  Vegetative cover that is established can be modified according to the property owner’s wishes.

C. Soil Fertility Application

Based upon the soil test results, variations of the following will be broadcast on the soil surface and incorporated immediately with tillage equipment.

1. 4-0-0-6 Calcium if necessary as determined by soil test at an approximate rate of 2-4 gallons per acre.

2. 8-3-3 plus Clean MicroBiotics applied at approximately 1-2 gallons per acre.

3. Mixtures of nitrogen, phosphorus and potassium.

4. Other nutrients and/or trace elements as necessary to remediate soils, so that crop production is possible.

IV. Maintenance Phase

The amount of maintenance required will vary with the soil and climatic conditions and the plant species that are being established.  The following factors are considered:

A. Soil Texture

Coarse textured soils with low organic matter content will require regular water application and nutrient applications until vegetation is well rooted and established.

B. Rainfall Patterns

Heavy rainfall periods may leach nutrients out of the rooting zone.  This is particularly problematic on coarse soils of low organic matter content.  Additional nutrient applications may be necessary on semi-annual basis.

C. High Water Tables

Water moves in the soil by capillary action and is not influenced by gravity until water levels reach field capacity.  A prolonged warm dry period following an unusually wet period could cause contaminates to move into the rooting zone from an untreated area adjacent to the treated area, or from subsoil below the area where bioremediation is complete.  In the event this did occur, the bioremediation phase would need to be repeated.

D. Other Considerations

1. In the event soil pH is very high or very low prior to the recolonization of biological life in the soil, a foliar feeding program would be required to provide certain trace elements that allow the vegetation to grow properly until biological life in the soil normalizes soil pH.

2. The soil microbe populations that are used to remediate hydrocarbons include species that are aerobic.  This means they require oxygen to survive and carry out their function.  Some soils will require mechanical aeration to keep the oxygen to carbon dioxide ratios at proper levels.

3. Every contamination should be assessed on individual basis. If unusual circumstances are suspected or confirmed, specific treatment procedures - both application and products - should be designed to assure successful remediation.

Post Application Phase

• Soil samples should be taken at regular intervals throughout the bioremediation and post application to determine when site reaches acceptable limits    
• Soil moisture must be maintained throughout the bioremediation phase

Post Application Phase

• Soil samples should be taken at regular intervals throughout the bioremediation and post application to determine when site reaches acceptable limits    
• Soil moisture must be maintained throughout the bioremediation phase

Application Guidelines for Waste Water Treatment: